S.E. Postnov2, V.A. Zuev 1, T.G. Borovaya 1
FSBI «National Research Centre for Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya» of the Ministry of Health of the Russian Federation, Moscow, Russia.2FSUE «The Central Aerohydrodynamic Institute named after Prof. N.E. Zhukovsky», Zhukovsky,
Abstract
The article presents the results of eight years of comprehensive experimental research conducted at the National Research Centre for Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya» of the Ministry of Health of the Russian Federation.
During the experiment, a methodology for active regulation control of homeostasis parameters was developed and tested on three groups of mice with different health conditions and severe pathology.
The obtained results of experimental studies confirm the extension fact of prolongation of the active phase of life of mice (the so-called “youth phase”) and, accordingly, an increase in life expectancy in general due to active control of physical parameters of homeostasis.
During the work, signs Throughout the study, indicators of youth in mammals were formulated, and their preservation in the experimental group of mice was confirmed experimentally empirically.
The active control method regulation technique of homeostasis has proven demonstrated effectiveness when working with applied to volunteers, restoring homeostasis parameters within a few minutes.
A characteristic example A representative case of homeostasis restoration in a patient with an acute inflammatory process in both kidneys, bilateral kidney inflammation complicated by high fever, is provided as an illustration example.
The following criteria were adopted for preserving to assess the preservation of the youth phase in mice:
- the state of the brain, characterized by the level of the aging factor in blood serum;
- the state of the reproductive function and the structure of some internal organs (based on histological analysis) in experimental mice at advanced stages of life, by which time the mice in the control groups had already passed away;
- fertility in experimental mice at later stages of life.
The results of the studies provide comparative data on the level of the aging factor in the blood serum of control and experimental animals, as well as average and maximum life expectancy, the birth of offspring at late stages of life, and the histological analysis of internal organs in 28-month-old mice from the experimental group, which significantly outlived the control group animals.
The histological analysis of the lungs, heart, cerebral cortex, ovaries, lymph nodes, and thymus confirmed the preservation of structural and functional characteristics of these organs in the experimental group animals. The ovarian histology explained the continued reproductive activity of the experimental group and the occurrence of offspring even at advanced stages of life.
While the above-mentioned results were anticipated during the experimental planning and execution, the presence of thymic morphology characteristic of young animals at advanced ages in the experimental mice is an important and unexpected finding that warrants further investigation.
This research validates the scientific direction focused on discovering methods for the active regulation of homeostasis parameters to extend the organism’s biologically active phase, namely, youth, and, consequently, to prolong the overall lifespan of the organism.
Idea of the Work /1/:
Hypothesis 1: Within a living organism, the fluids forming the basis of intercellular and intracellular fluids, plasma and lymph, are chemically H₂O, yet their physical properties differ fundamentally from those of drinking water. For example, physiological saline, which closely mimics the chemical composition of blood plasma, can only be intravenously administered in strictly limited amounts and at a controlled rate, due to its significant physical disparity from blood plasma.
Moreover, within the body, the physical parameters of fluids are maintained in a non-equilibrium state, requiring constant effort from the organism to keep them within the so-called physiological norm, a phenomenon referred to as homeostasis in biology and medicine.
Hypothesis 2: The physical characteristics of homeostasis serve as the foundation for its biochemical parameters. Therefore, maintaining these physical parameters actively within the physiological corridor substantially aids the organism in stabilizing its biochemical homeostasis and reduces the overall energy expenditure required to do so.
Hypothesis 3: Active regulation of the physical parameters of homeostasis, ensuring they remain within physiological limits throughout all stages of life, including under adverse internal and external influences, allows the organism to conserve energy and biological resources during its lifespan.
If these hypotheses hold true, then active homeostasis control enables the body’s cells to exist in a “gentle mode.” This would allow the cells to complete their life cycles in the time naturally allotted to them. As a result, cell divisions in the experimental group occur less frequently compared to the control group. Given the biological limit on cell division (e.g., telomere shortening with each division), cells—and therefore organs—in the experimental group would not only live longer but also retain youthful characteristics for a significantly extended period.
To test these hypotheses, a direct comparative experiment was conducted using laboratory animals. This involved recording average and maximum lifespan in control and experimental groups, monitoring biological indicators of youth, and conducting histological analyses of organs from experimental animals at later stages of life.
Objective of the Work:
To experimentally confirm, using laboratory animals:
- the possibility of actively regulating homeostasis parameters;
- the potential to extend the youth phase of the mammalian organism;
- the achievement of increased life expectancy in general;
- the validation and explanation of findings through histological analysis of internal organs.
Prerequisites for the Study:
This study is based on a “Biophysical Model of a Living Organism” [1], constructed as a system of hypotheses. One implication of this model is the conclusion that the youth phase can be extended by creating conditions that allow cells, in the intervals between divisions, to complete their full natural life cycles.
This will result in a prolongation of their active lifespan, and consequently, an extension of the functional lifespan of organs and the organism as a whole, thus prolonging both the youth phase and overall life expectancy.
To achieve this, the biophysical model [1] proposes the use of:
- active homeostasis regulation, particularly through the monitoring and control of physical parameters of internal body fluids. Maintaining these parameters within the physiological corridor will enable biological and biochemical processes to proceed with maximum efficiency.
- active mitigation of negative factors affecting the body, by neutralizing and/or significantly reducing the harmful consequences caused by their presence. For example, notably decreasing the viral load on the body.
This proposal is based on Hypothesis [1], which suggests that the combined effect of negative factors increases nonlinearly with the number of such factors. Therefore, a reduction in their number would also nonlinearly diminish the overall damage they inflict on the body.
Research Objects and Tools for Achieving the Goal:
Laboratory animals: linear mice, laboratory debilitated mice, short-lived diabetic mice, human cell cultures, recruited volunteers.
The drug with the working name D120 Patent No. 2615 /2/, the use of which allows for active control of homeostasis.
The drug with the working name D120 (Patent No. 2615) /2/, was used to actively regulate homeostasis.
The drug D120 (hereinafter referred to as the “Preparation”) also exhibits a range of distinct biological effects, as revealed through the conducted research [4]. These include:
- pronounced antiviral activity,
- the ability to enhance the immune system, particularly cellular and humoral immunity, and
- the capacity to reduce the synthesis rate of the aging factor, the level of which influences the rate of neuronal death in the brain.
Additionally, the Preparation has shown efficacy in reducing the negative consequences of various harmful factors affecting the body. Based on these demonstrated capabilities, the Preparation was selected as the principal tool for achieving the goals of this study.
Example of Preparation D120 Functioning in the Human Body
The prerequisites for this research also include numerous experiments involving human volunteers, during which the physical parameters of internal fluids and general homeostasis were monitored before and after administration of the Preparation, using a variety of diagnostic equipment.
These studies were carried out only after receiving validated data confirming the safety of the Preparation, including its long-term effects. Safety assessments were performed in accordance with the principles of evidence-based medicine /4,6/, and included tests on human cell cultures, laboratory animals, and primates.
To illustrate the active control of physical homeostasis parameters in humans, we present the results of an assessment conducted by Professor A.I. Krashenyuk, using the “Aquafon” diagnostic system (Registration Certificate No. FSR 2010/07292) [3]. This case is particularly illustrative, as it demonstrates how the Preparation enabled the rapid normalization of physical homeostasis parameters that had been outside the physiological corridor—achieving restoration within a matter of minutes.
The diagnostic system used in this study enables real-time monitoring of subtle physical parameters, such as the re-emission of electromagnetic pulses of a specified frequency by the internal fluids of the human body.
Figure 1 (left) presents the disrupted physical parameters in a 24-year-old patient suffering from acute bilateral kidney inflammation, accompanied by a high fever.
Figure 1 (right) shows how these parameters returned to physiological norms just 20 minutes after ingestion of the Preparation.
Color-Based Assessment of Internal Fluid Parameters
The diagnostic color palette used to evaluate the physical parameters of human internal fluids is interpreted as follows: green, blue, and cyan shades correspond to normal physiological conditions, while yellow, red, and brown indicate deviations beyond the physiological norm.
Research Findings on Preparation D120’s Capability to Mitigate Harmful Biological Impacts
Before formal implementation of the stated objectives, a series of extensive experiments were conducted at the National Research Centre for Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya to evaluate the biological impact of Preparation D120.
Researchers M.V. Mezentseva and R.Ya. Podchernyaeva, through a comprehensive investigation [4], discovered that Preparation D120:
- Reduced the toxicity of commonly used cell culture components such as cattle serum and fetal calf serum;
- Did not alter the viability or proliferative activity of normal human cell lines (4 lines tested);
- Inhibited the growth and proliferative activity of cancer cell lines (6 tested), with 3 lines completely degrading after the first administration.
These outcomes align with findings from N.M. Anichkov and his team [5], who established that physical parameters of intracellular fluid—particularly optical density—differ between normal and cancer cells. It is hypothesized that Preparation D120 restores these parameters to physiological norms, thereby restricting or halting cancer cell metabolism.
Furthermore, the administration of D120 was shown to influence the cytokine profiles of T1 and T2 cells, enhancing their immune responsiveness [4].
In in vitro studies, Preparation D120 exhibited potent antiviral activity. When combined with a known interferon inducer, it amplified the antiviral response and nearly eliminated influenza A virus replication. The authors suggest that this effect is mediated through the expression of cytokine genes in T- and B-lymphocytes under D120’s influence [4].
The development of these studies on the experimental influenza model in vivo (on mice) allowed confirmation of the antiviral activity of the drug D120 and determination of the efficiency index equal to 40%. It was also established that in vivo, the use of D120 promotes a decrease in the transcriptional synthesis of cytokines that influence the formation of humoral immunity (IL-4 and IL-6), the increased synthesis of which is an indicator of the chronicity of infection. But at the same time—and importantly—D120 causes the normalization of the production of IL-2 and IL-12 cytokines at the transcriptional level, which are associated with the mechanisms of increased protection against infection /4/.
Continuation of these studies also revealed the antiviral activity of the drug D120 against herpes simplex viruses types 1 and 2, as well as the complete inactivation of the mouse encephalomyocarditis virus. It is characteristic that in the experimental herpes infection in the in vivo system caused by the herpes simplex virus type 1, the efficiency index was 43%. When determining the cytokine-modulating activity of D120, its ability to cause this precise regulatory effect on the synthesis of mRNA of certain cytokines was revealed—cytokines that increase the efficiency of the immune defense of animals in relation to the herpes virus /4/.
The studies of the antiviral activity of the drug D120 were expanded by including two groups of volunteers who had been taking the drug for a long time under observation. The first group consisted of 22 athletes aged 18–21, the second of 12 people—professors and teachers of Moscow universities aged 68–79. For 6 months, the volunteers were monitored for the restoration of blood parameters, including blood cells responsible for immunity. During the observation, it was discovered that 25% of the volunteers were carriers of the SV40 oncovirus. After 6 months of using the drug D120, the virus was no longer detected in their blood.
Coincidentally, similar studies of the antiviral activity of the Preparation on the SV40 oncovirus were conducted at the Research Institute of Medical Primatology on monkeys and with the involvement of volunteers /6/. Cycloferon was used as a comparison drug. After 2 months of administration, the effectiveness of the drug D120 on the SV40 virus in humans was 71.4%, and that of cycloferon was 50%. In monkeys, after 3 months of administration, the effectiveness of the drugs D120 and cycloferon was the same. It was also recorded that administration of the drug D120 led to an improvement in the general clinical condition of the monkeys. In particular, the gastrointestinal tract function was normalized, and in monkeys dying from a systemic autoimmune process, an improvement in the general clinical condition was observed /6/.
Aging factor
In 2003, V.A. Zuev suggested that the well-known processes of damage and death of neurons during aging are secondary and caused by active proliferation of glial cells. To launch such a proliferative process in the aging brain, a certain factor—an “aging factor”—must accumulate, stimulating the development of gliosis /7/. Further studies by V.A. Zuev and co-authors confirmed the correctness of this assumption. Moreover, the increase in the rate of death of neurons in the brain with age is associated with an increase in the rate of synthesis of the aging factor with age and its accumulation in the brain /8–12/.
Therefore, the level of the aging factor in the blood serum of mice, where it also accumulates with age, was taken as a criterion for the youth of the brain.
In previous studies on the effect of D120 on the level and activity of the aging factor, it was clearly established that the introduction of the aging factor to mice leads to a decrease in the number of neurons in the somatosensory cortex, as well as satellite glia, compared to the control. The number of free and total glia, and the glioneuronal index, did not change.
Analysis of the effect of the drug D120 following the introduction of the aging factor revealed a completely different picture. The administration of Preparation D120 to mice that were previously administered the aging factor was accompanied by an increase in the number of neurons compared to animals that were administered only the aging factor. At the same time, the number of satellite and total glia also increased.
The glioneuronal index in these mice did not change. Thus, in animals that were administered the drug D120 against the background of the aging factor, not only did neurons not suffer and reactive gliosis not develop, but they even exhibited all the signs of improved neuronal nutrition.
The obtained preliminary results clearly indicate the safety of the drug D120, its anti-infective and anti-cancer activity, its positive effect on the organs and systems of the body, and finally, the similarity of the properties of the drug D120 to the fluid in the body itself.
That is, the use of the drug D120 allows for a significant reduction in the number and severity of adverse factors affecting a living organism.
Results of the experiment
At the National Research Centre for Epidemiology and Microbiology named after Honorary Academician N. F. Gamaleya, after a series of short-term determinations of mammal survival and preliminary experiments, a long-term 8-year study of the effect of the drug D120 preparation on the lifespan of mammals was undertaken under the supervision of V.A. Zuev. The study assessed the level of the aging factor in the blood serum, fertility, survival times, and the average and maximum lifespan of each individual.
Three groups of mice were used in the study:
- The first group consisted of outwardly healthy 6-month-old C57Black/6 mice with a lifespan of about 2 years.
- The second group consisted of outwardly healthy 3-month-old C57Black/6 mice with a lifespan of about 1 year (debilitated).
- The third group consisted of short-lived mutant mice of the B/KS-db+/+m line, representing a genetic model of type 2 diabetes mellitus suitable for evaluating new methods of treatment in an experiment. This model reproduces the characteristic stages of diabetes mellitus type 2: in the early stages (1–2 months), tissue resistance to insulin develops and is compensated by hypertrophy of islet cells; at 3–4 months, the stage of true insulin deficiency develops, as in diabetes mellitus type 1; and at 5–6 months, the terminal stage of diabetes mellitus develops, leading to profound metabolic disorders, cachexia, and death.
Animals of the 1st group—healthy 6-month-old mice of the C57Black/6 line—were divided into 3 subgroups of 10 individuals each.
- Animals of the 1st subgroup received 25 μl of Preparation per os daily for 2.5 months.
- Animals of the 2nd subgroup received Preparation until the end of their lives.
- Ten mice served as controls.
Observation of animals in the 1st group continued for almost 5 years. In the second year of life, a noticeable decrease in the levels of the aging factor in the blood serum of mice in both experimental subgroups was detected compared to the control animals. Moreover, in animals of the 2nd subgroup, which took Preparation constantly, this decrease was more pronounced (Fig. 2).
The onset of mortality in animals began on the 513th day in the control group, and occurred later in the first experimental subgroup, where the subsequent mortality rate slowed down. This trend was reflected in both the average and maximum lifespan indicators (see Fig. 3 and Table 1). A particularly noteworthy pattern of mortality was observed in the second experimental subgroup, in which the first deaths were recorded only after the animals reached an age exceeding 3.7 years (Fig. 3).
As demonstrated by the data presented in Table 1, the average lifespan of animals that received Preparation D120 for only 2.5 months was 28% greater than that of the control group. In contrast, the long-term administration of the drug to animals in the second experimental subgroup resulted in an increase in the average lifespan by 130%, effectively more than doubling it.
Table 1. Average and maximum lifespan of mice in groups
| Subgroup | The number of days each of the 10 mice lived | Sum of days | Life expectancy (days) | |
| Average | Maximum | |||
| Control | 513, 549, 598,598, 698, 708,708, 751, 793, 803 | 6719 | 671,9100% | 803100% |
| 1st subgroup | 537, 642, 698, 793, 880, 880, 897, 1018, 1135, 1157 | 8637 | 863,7+2+28% | 1157+44% |
| 2nd subgroup | 1376, 1376, 1382, 1517,1573, 1573, 1598, 1630, 1670, 1762 | 15457 | 1545,7+130% | 1762+119% |
The group of so-called “debilitated” mice of the C57Black/6 line, characterized by a naturally shorter lifespan, was divided into two subgroups—control and experimental—each comprising 13 individuals. Unlike the control subgroup, the mice in the experimental group received 0.25 μl of Preparation D120 orally on a daily basis. As anticipated, the onset of mortality in the control mice occurred as early as the second or third month of the first year of life. Notably, none of the control animals survived to the completion of the first year. In contrast, mice from the same genetic line in the experimental subgroup exhibited a delayed onset of mortality, with deaths beginning only in the fifth month of life. Furthermore, mortality in this group occurred over a prolonged interval, and several experimental mice survived for nearly two years (Fig. 4).
Based on the obtained data, the average and maximum lifespan of “weakened” mice receiving the drug D120 was higher than that of control mice by 67.3% and 100.6%, respectively (Table 2).
The final group—comprising short-lived diabetic mice, commonly referred to as a genetic model of type 2 diabetes mellitus—was acquired at 3 months of age and divided equally into experimental and control subgroups, with 16 individuals in each. In contrast to the controls, mice in the experimental subgroup received 0.25 μl of the D120 preparation orally on a daily basis. Additionally, in consideration of the severity of their pathological condition, a second container (250 ml) of regular tap water, into which 2.5 ml of D120 preparation had been pre-mixed, was placed in their cages at the end of each working day to ensure supplemental intake. As anticipated, mortality among the control group (Fig. 5) commenced from the fourth month of life and was characterized by a rapid progression. Predictably, all control animals succumbed before reaching 6 months of age.
At the same time, the death of mice treated with D120 was also characterized by a rather “steep” dynamics, but the time of their death occurred later and the process of gradual death itself lasted noticeably longer compared to the control (Fig. 5). These differences are expressed in the values of the average and maximum lifespan of animals (Table 3). Thus, due to the use of D120, the average and maximum lifespan of diabetic mice increased, respectively, by 31.5% and 95.7% compared to the control.
In Figure 3, asterisks mark the occurrence and timing of offspring births in the 1st and 2nd experimental subgroups of the 1st group (comprising healthy animals), occurring after 3 years of age, that is, near the conclusion of the observation period. Offspring were recorded once in the 1st experimental subgroup and twelve times in the 2nd experimental subgroup (Fig. 3, asterisks). The total number of offspring in the 1st experimental subgroup was 7, whereas in the 2nd experimental subgroup it reached 96. In contrast, in the control group, all animals had perished long before, and no offspring were recorded.
These findings—reduced levels of the aging factor, increased average and maximum lifespan, and enhanced reproductive capacity in animals administered the D120 preparation—strongly indicate a significant extension of the active, functional lifespan of mammals under the influence of D120.
Further insight into these results was gained through a morphological analysis of selected internal organs of experimental mice that had received D120 and significantly outlived their control counterparts. At the time of examination, the age of the experimental animals was 28 months, whereas animals in the control group had long since died.
The histological findings revealed the following:
- The cytoarchitecture of the cerebral cortex in experimental mice remained generally well-preserved. However, some destructive alterations were observed in the cells of the paraventricular nuclei, along with a reduction in the thickness of the granular layer of the cortex.
- The structure of the pulmonary acini and intrapulmonary bronchi in experimental animals (Fig. 6a, b) corresponded to physiological norms, exhibiting only minor signs of vascular hyalinosis.
Fig. 6a. Fig. 6b.
Fig. 6a, 6b. Fragments of the lung of an experimental mouse. Physiological structure of pulmonary acini and intrapulmonary bronchi. Fig. 6a, 6b: Hematoxylin and eosin staining. Magnification Fig. 6a, 6b: × 200.
The histological structure of the heart membranes of experimental mice corresponds to the norm (Fig. 7) with local signs of cardiomyocyte hypertrophy
Fig. 7. Fragment of the heart wall of an experimental mouse. Hematoxylin and eosin staining. Magnification: ×200.
4. In the structure of the lymph nodes of the experimental mice, lymphoid follicles are clearly represented (Fig. 8a); in the structure of the thymus – the cortex and medulla (Fig. 8b).
Fig. 8a. A fragment of the lymph node from Fig. 8b – A fragment of the thymus lobe from
an experimental mouse an experimental mouse
Fig. 8a, 8b – Hematoxylin and eosin staining. Magnification: Fig. 8a, 8b: × 200.
he ovaries of experimental mice contain: follicles at different stages of development (Fig. 9a, b), large cystic follicles, and corpora lutea.
Fig. 9a, Fig. 9b
A fragments of ovaries of experimental mice containing ovarian follicles at different stages of development. Fig. 9a, 9b – hematoxylin and eosin staining. Magnification: Fig. 9a × 200; 9b ×400
The obtained general histological assessments of the internal organs of the experimental mice suggest that these organs remained in a functionally competent state and likely continued to fulfill their physiological roles throughout the animals’ extended lifespan, despite their advanced age.
It can thus be concluded that D120 contributes to the preservation of both the structural and functional integrity of the lungs and heart, supports ovofolliculogenesis, and enables the continuation of reproductive activity even in the later stages of the female lifespan.
The histological analysis conducted on the cerebral cortex of the experimental group confirmed an overall satisfactory morphological condition, which aligns with the marked reduction in the level of the aging factor observed in the blood plasma, relative to control animals.
Furthermore, D120 exhibits a pronounced immunomodulatory effect, as evidenced by the preserved histoarchitecture of the lymph nodes and the continued presence of the thymus in treated animals.
Discussion of the Results
To summarize the presented material, it should be noted that the very fact of an increase in life expectancy under the influence of the drug D120 was expected. However, its quantitative effect was unexpected. Moreover, the use of D120 contributed to an extension of the active phase of life in animals, and this was observed across all three lines of mice, which differed significantly in their health status: healthy linear mice, debilitated laboratory mice, and short-lived mice with serious congenital pathologies. At the same time, it is worth noting that the maximum life expectancy, as a percentage relative to the control group, was similar across different mouse groups. However, the percentage increase in average life expectancy was strongly dependent on the initial health condition of the mice.
The extension of the active phase of life (interpreted as a prolongation of youth) was experimentally confirmed by several indicators: a significant decrease in the amount of the aging factor in blood plasma, the birth of offspring at late life stages—at a time when control mice had already died—and the preservation of immune system organs, including the thymus. Therefore, the use of the D120 preparation makes it possible to preserve at least three signs of youth:
- Youth of the brain, biologically marked by a reduced rate of aging protein synthesis and, accordingly, a slower rate of neuronal death in the brain, which typically increases with age;
- Youth of the reproductive system /13/, as evidenced by the birth of numerous offspring in the later stages of life;
- Youth of the immune organs, with clear evidence of preserved structural integrity.
It should be especially emphasized that the morphological structure of the thymus, characteristic of young animals and identified in 28-month-old mice from the experimental group, represents a noteworthy result of this experiment and requires further study.
Summarizing the above, it can be stated that it has been experimentally demonstrated:
- That the hypothesis of the “Biophysical model” /1/ is valid. This model states that an increase in the life expectancy of a living organism, including humans, and an extension of the active phase of life, can be achieved through the monitoring and regulation of homeostasis in terms of its physical parameters.
- Moreover, the results obtained form the basis for a new direction in gerontology. In addition to the current focus on “studying the signs of aging and developing methods to combat them,” there is now the potential to develop a new approach: “studying and classifying the signs of youth and developing methods to extend them.”
To summarize the above, it can be stated that it has been experimentally shown:
- The hypothesis of the “Biophysical model” /1/ has a right to exist, stating that an increase in the life expectancy of a living organism, including humans, and an extension of the active phase of its life, can be achieved by monitoring and control of homeostasis in terms of its physical parameters.
- Furthermore, the results create the prerequisites and hope for a new direction in gerontology: along with the existing trend of “studying the signs of aging and developing ways to combat them,” there is potential to form a new direction—”studying and classifying the signs of youth and developing means and methods to extend them.”
Acknowledgment
In conclusion, the authors consider it their duty to express deep gratitude to the Director of the National Research Center of Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation, Academician of the Russian Academy of Sciences Alexander Leonidovich Ginzburg, for his long-term interest in the subject under study and continuous support for the research conducted in this cycle.
Literature.
1. Postnov S.E. The role of boundary layer water in a living organism. Biophysical model// Biomedical radio engineering. 2008, № 12, P. 52-56.
2. Postnov S.E. Method for obtaining medicinal and biologically active agents. Patent № 2479318, 2012г.
3. Diagnostic complex “Aquafon”, registration certificate №FSR No. 2010/07292).
4. Postnov S.E., Mezentseva M.V., Podchernyaeva R.Ya., Danlybaeva G.A., Surgucheva I.M., Grinkevich O.M., Lopatina O.A., Baklanova O.V. New approaches in biomedical technology based on water from the boundary layer//Biomedical Radioelectronics.2009, №1, P.3-15.
5. Anichkov N.M., Khaloimov A.I., Rozin I.T. Changes in the state of water in hydrolysis and tissue fluids in malignant tumors//Medical Academic Journal. 2005, Vol. 5, №3, P. 35-43.
6. Mezentseva M.V., Agrba V.Z., Postnov S.E., Karal-Ogly D.D., Shapoval I.M., Afanasyev N.A., Russu L.I., Agumava A.A., Lapin B.A. Peculiarities of expression of regulatory cytokine genes in monkeys carrying SV-40//Fundamental and applied aspects of medical primatology. 2011, Vol. 1, P. 93-100.
7. Zuev V.A., Ignatova N.G. Patent №2204135 dated May 10, 2003
8. Zuev V.A., Viktorov I.V., Borodina N.P., Ignatova N.G., Bykovskaya S.I., Khalansky A.S. Possible role of proliferative activity of glia in damage and death of neurons during aging//In the collection: “Alzheimer’s disease and aging: from neurobiology to therapy” Proceedings of the II Russian conference. Moscow, October 18-20, 1999. Ed. S.I. Gavrilova. Publishing house “Pulse” Moscow. -1999. – P. 45-51.
9. Zuev V.A., Viktorov I.V., Borodina N.P., Ignatova N.G., Bykovskaya S.I., Khalansky A.S. Accumulation in the aging mammalian brain of a factor that sharply stimulates the proliferative activity of glia//Bulletin of Experimental Biology and Medicine, 2000, №3, P. 317-320.
10. Zuev V.A., Avtandilov G.G., Ignatova N.G., Bykovskaya S.I. Artificial aging of mice// Bulletin of Experimental Biology and Medicine, 2003, № 9, P. 325-327.
11. 1. Zuev V.A., Ignatova N.G., Avtandilov G.G. Accumulation of the aging factor in the body of mammals, including humans//Advances in Gerontology, 2005, Vol. 17, P. 108-116.
12. Zuev V.A., Varfolomeev S.D., Gribov L.A., Nikolaev E.N., Sedykh E.M., Ignatova N.G., Klyushnik T.P., Shaposhnikova G.M., Samoilenko I.I., Kurova V.S. Biological and physicochemical properties of the aging factor in mammals//Clinical gerontology, 2006, Vol. 12, №9, P. 87.
13. Postnov S.E., Zuev V.A. Patent №2615154 Method for increasing the average and maximum lifespan and fertility of mammals, 2016.
